Associate Professor
Genetics and biochemistry of vitamin B12
Selected Publications
Kuhnl, J., Bobik, T.A., Procter, J.B., Burmeister, C., Hoppner,
J., Wilde, I., Luersen, K., Torda, A.E., Walter, R.D. and Liebau, E.
(2005) Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis
elegans. FEBS J. 272, 1465-1477.
Sampson, E., Johnson, C.L.V. and Bobik, T.A. (2005) Biochemical evidence
that the pduS gene encodes a cobalamin reductase. Microbiology 151,
1169-1177.
Leal, N.A. and Bobik, T.A. (2004) Human ATP:cob(I)alamin adenosyltransferase
and its interaction with methionine synthase reductase. J. Biol.
Chem. 279, 47536-47542.
Sheppard, D., Penrod, T. J., Bobik, T.A., Kofoid, E. and Roth, J.R.
(2004) Evidence that a B12-adenosyltranferase is encoded within the
ethanolamine operon of Salmonella enterica. J. Bacteriol. 186,
7635-7644 .
Johnson, C.L.V., Buszko, M.L. and Bobik, T.A. (2004) Purification and
initial biochemical characterization of the Salmonella enterica PduO
ATP:cob(I)alamin adenosyltransferase. J. Bacteriol.186, 7881-7887
.
Bobik, T.A. and Rasche, M.E. (2004) Purification and partial characterization
of the Pyrococcus horikoshii methylmalonyl-CoA epimerase. Appl.
Microbiol. Biotechnol. 63, 682-685.
Leal, N.A., Havemann, G.D. and Bobik, T.A. (2003) The PduP protein is
a propionaldehyde dehydrogenase associated with polyhedral organelles
involved in coenzyme B12-dependent 1,2-propanediol degradation by Salmonella
enterica. Archives Microbiol. 180, 353-361.
Havemann, G.D. and Bobik, T.A. (2003) Protein content of the polyhedral
organelles involved in coenzyme B12-dependent degradation of 1,2-Propanediol
in Salmonella enterica Serovar Typhimurium LT2. J. Bacteriol.
185, 5086-5095.
Leal, N.A., Park, S.D., Kima, P.E. and Bobik, T.A. (2003). Identification
of the human and bovine ATP:Cob(I)alamin adenosyltransferase cDNA based
on complementation of a bacterial mutant. J. Biol. Chem. 278,
9227-9234.
Bobik, T.A. and Rasche, M.E. (2003). HPLC Assay for Methylmalonyl-CoA
Epimerase. Anal. Bioanal. Chem. 375, 344-349.
Havemann G.D, Sampson, E. and Bobik, T.A. (2002) The PduA protein is
a component of the shells of polyhedral organelles formed by Salmonella
during B12-dependent growth on 1,2-propanediol. J. Bacteriol.
184, 1253-1261.
Bobik, T.A. and Rasche, M.E. (2001) Identification of the human methylmalonyl-CoA
racemase gene based on the analysis of prokaryotic gene arrangements:
implications for decoding the human genome. J. Biol. Chem.
276, 37194-37198.
Johnson, C.L.V., Pechonick, E., Park, S.D., Havemann, G.D., Leal, N.A.,
and Bobik, T.A. (2001) Functional genomic, biochemical and genetic characterization
of the Salmonella pduO gene, an ATP:cob(I)alamin adenosyltransferase
gene. J. Bacteriol. 183, 1577-1584.
Price-Carter, M., Tingey, J., Bobik, T.A. and Roth, J.R. (2001) The
alternative electron acceptor tetrathionate supports B12-dependent anaerobic
growth of Salmonella enterica serovar Typhimurium on ethanolamine or
1,2-propanediol. J. Bacteriol. 183, 2463-2475.
