Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology > Gustavo MacIntosh

Gustavo MacIntosh

8 x 10 in

Associate Professor

  • Gene expression and metabolic changes during plant defense responses to pests
  • Functional genomics of plant nucleases

4108 Molecular Biology Building
Dept. of Biochemistry, Biophysics & Molecular Biology
Iowa State University
Ames, IA 50011

Phone:  (515) 294-2627


Ph.D., Biological Chemistry, University of Buenos Aires, 1997
Postdoctoral Fellow, Michigan State University, 1998-2001

Research Interests

My laboratory is interested in the interaction between plants and insects. In particular, we study how plants respond to insect attacks, and the mechanisms used by insects to avoid plant defenses and to take over plant metabolism. We are also interested in the role of secreted ribonucleases in these defense interactions.

Our current projects are:

  • Characterization of the transcriptional and metabolic changes induced in soybean by the soybean aphid, Aphis glycines.
  • Role of general stress responses in the interaction between plants and aphids.
  • Identification of novel signaling pathways that control defense responses to insects and mechanical wounding in Arabidopsis and soybean.
  • Biological roles of secreted ribonucleases in defense responses and also in other cellular processes.

Please visit the lab web site for more information

Selected Publications

1:  Floyd BE, Morriss SC, Macintosh GC, Bassham DC. What to eat: evidence for selective autophagy in plants. J Integr Plant Biol. 2012 Nov;54(11):907-20. doi: 10.1111/j.1744-7909.2012.01178.x. PubMed PMID: 23046163.

2:  Studham ME, MacIntosh GC. Multiple phytohormone signals control the transcriptional response to soybean aphid infestation in susceptible and resistant soybean plants. Mol Plant Microbe Interact. 2013 Jan;26(1):116-29. doi: 10.1094/MPMI-05-12-0124-FI. PubMed PMID: 22992001.

3:  Studham ME, MacIntosh GC. Phytohormone signaling pathway analysis method for comparing hormone responses in plant-pest interactions. BMC Res Notes. 2012 Jul 31;5:392. PubMed PMID: 22846705; PubMed Central PMCID: PMC3460778.

4:  McCarville MT, Kanobe C, MacIntosh GC, O’Neal M. What is the economic threshold of soybean aphids (Hemiptera: Aphididae) in enemy-free space? J Econ Entomol. 2011 Jun;104(3):845-52. PubMed PMID: 21735903.

5:  MacIntosh GC, Bassham DC. The connection between ribophagy, autophagy and ribosomal RNA decay. Autophagy. 2011 Jun;7(6):662-3. Epub 2011 Jun 1. PubMed PMID: 21460615.

6:  Hillwig MS, Contento AL, Meyer A, Ebany D, Bassham DC, Macintosh GC. RNS2, a conserved member of the RNase T2 family, is necessary for ribosomal RNA decay in plants. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1093-8. doi: 10.1073/pnas.1009809108. Epub 2011 Jan 3. PubMed PMID: 21199950; PubMed Central PMCID: PMC3024651.

7:  Hillwig MS, Kanobe C, Thornburg RW, Macintosh GC. Identification of S-RNase and peroxidase in petunia nectar. J Plant Physiol. 2011 May 1;168(7):734-8. doi: 10.1016/j.jplph.2010.10.002. Epub 2010 Nov 18. PubMed PMID: 21093100.

8:  Chiozza MV, O’Neal ME, MacIntosh GC. Constitutive and induced differential accumulation of amino acid in leaves of susceptible and resistant soybean plants in response to the soybean aphid (Hemiptera: Aphididae). Environ Entomol. 2010 Jun;39(3):856-64. doi: 10.1603/EN09338. PubMed PMID: 20550799.

9:  Hillwig MS, Liu X, Liu G, Thornburg RW, Macintosh GC. Petunia nectar proteins have ribonuclease activity. J Exp Bot. 2010 Jun;61(11):2951-65. doi: 10.1093/jxb/erq119. Epub 2010 May 11. PubMed PMID: 20460362; PubMed Central PMCID: PMC2892141.

10:  MacIntosh GC, Hillwig MS, Meyer A, Flagel L. RNase T2 genes from rice and the evolution of secretory ribonucleases in plants. Mol Genet Genomics. 2010 Apr;283(4):381-96. doi: 10.1007/s00438-010-0524-9. Epub 2010 Feb 25. PubMed PMID: 20182746.

11:  Hillwig MS, Rizhsky L, Wang Y, Umanskaya A, Essner JJ, MacIntosh GC. Zebrafish RNase T2 genes and the evolution of secretory ribonucleases in animals. BMC Evol Biol. 2009 Jul 20;9:170. doi: 10.1186/1471-2148-9-170. PubMed PMID: 19619322; PubMed Central PMCID: PMC2720953.

12:  Hillwig MS, Lebrasseur ND, Green PJ, Macintosh GC. Impact of transcriptional, ABA-dependent, and ABA-independent pathways on wounding regulation of RNS1 expression. Mol Genet Genomics. 2008 Sep;280(3):249-61. doi: 10.1007/s00438-008-0360-3. Epub 2008 Jul 8. PubMed PMID: 18607631.