Professor
Biochemistry and molecular biology of biotin and biotin-containing enzymes
Regulation of plant lipid metabolism
Selected Publications
Dietrich, C.R., Perera, M.A., M, D.Y.-N., Meeley, R.B., Nikolau, B.J.
and Schnable, P.S. (2005) Characterization of two GL8 paralogs reveals
that the 3-ketoacyl reductase component of fatty acid elongase is essential
for maize (Zea mays L.) development. Plant J. 42, 844-861.
Fatland, B.L., Nikolau, B.J. and Wurtele, E.S. (2005) Reverse genetic
characterization of cytosolic acetyl-CoA generation by ATP-Citrate lyase
in Arabidopsis. Plant Cell 17, 182-203.
Yandeau-Nelson, M.D., Zhou, Q., Yao, H., Xu, X., Nikolau, B.J. and Schnable,
P.S. (2005) MuDR transposase increases the frequency of meiotic crossovers
in the vicinity of a Mu insertion in the maize a1 gene. Genetics
169, 917-929.
Sluszny, C., Yeung, E.S. and Nikolau, B.J. (2005) In-situ probing of
the biotic-abiotic boundary of plants by laser desorption/ionization
time-of-flight mass spectrometry. J. Am. Soc. Mass. Spectrom.
16, 107-115.
Bino, R.J., Hall, R.D., Fiehn, O., Kopka, J., Saito, K., Draper, J.,
Nikolau, B.J., Mendes, P., Roessner-Tunali, U., Beale, M.H., Trethewey,
R.N., Lange, B.M., Wurtele, E.S. and Sumner, L.W. (2004) Potential of
metabolomics as a functional genomics tool. Trends Plant Sci.
9, 418-425.
Nikolau, B.J., Ohlrogge, J.B. and Wurtele, E.S. (2003) Plant biotin-containing
carboxylases. Arch. Biochem. Biophys. 414, 211-222.
Che, P., Weaver, L.M., Wurtele, E.S. and Nikolau, B.J. (2003) The role
of biotin in regulating 3-methylcrotonyl-coenzyme a carboxylase expression
in Arabidopsis. Plant Physiol. 131, 1479-1486.
Yao, H., Zhou, Q., Li, J., Smith, H., Yandeau, M., Nikolau, B.J. and
Schnable, P.S. (2002) Molecular characterization of meiotic recombination
across the 140-kb multigenic a1-sh2 interval of maize. Proc. Natl.
Acad. Sci. USA 99, 6157-6162.
Xu, X., Dietrich, C.R., Lessire, R., Nikolau, B.J. and Schnable, P.S.
(2002) The Endoplasmic reticulum-associated maize GL8 protein is a component
of the acyl-coenzyme A elongase ivolved in the production of cuticular
waxes. Plant Physiol. 128, 924-934.
Oliver, D.J., Nikolau, B. and Wurtele, E.S. (2002) Functional genomics:
high-throughput mRNA, protein, and metabolite analyses. Metab. Eng.
4, 98-106.
Fatland, B.L., Ke, J., Anderson, M.D., Mentzen, W.I., Cui, L.W., Allred,
C.C., Johnston, J.L., Nikolau, B.J. and Wurtele, E.S. (2002) Molecular
characterization of a heteromeric ATP-citrate lyase that generates cytosolic
acetyl-coenzyme A in Arabidopsis. Plant Physiol. 130, 740-756.
Dietrich, C.R., Cui, F., Packila, M.L., Li, J., Ashlock, D.A., Nikolau,
B.J. and Schnable, P.S. (2002) Maize Mu transposons are targeted to
the 5' untranslated region of the gl8 gene and sequences flanking Mu
target-site duplications exhibit nonrandom nucleotide composition throughout
the genome. Genetics 160, 697-716.
Che, P., Wurtele, E.S. and Nikolau, B.J. (2002) Metabolic and environmental
regulation of 3-methylcrotonyl-coenzyme A carboxylase expression in
Arabidopsis. Plant Physiol. 129, 625-637.
Wurtele, E.S. and Nikolau, B.J. (2000) Characterization of 3-methylcrotonyl-CoA
carboxylase from plants. Methods Enzymol. 324, 280-292.
Nikolau, B.J., Oliver, D.J., Schnable, P.S. and Wurtele, E.S. (2000)
Molecular biology of acetyl-CoA metabolism. Biochem. Soc. Trans.
28, 591-593.
McKean, A.L., Ke, J., Song, J., Che, P., Achenbach, S., Nikolau, B.J.
and Wurtele, E.S. (2000) Molecular characterization of the non-biotin-containing
subunit of 3-methylcrotonyl-CoA carboxylase. J. Biol. Chem.
275, 5582-5590.
Ke, J., Wen, T.N., Nikolau, B.J. and Wurtele, E.S. (2000) Coordinate
regulation of the nuclear and plastidic genes coding for the subunits
of the heteromeric acetyl-coenzyme A carboxylase. Plant Physiol.
122, 1057-1071.
Ke, J., Behal, R.H., Back, S.L., Nikolau, B.J., Wurtele, E.S. and Oliver,
D.J. (2000) The role of pyruvate dehydrogenase and acetyl-coenzyme A
synthetase in fatty acid synthesis in developing Arabidopsis seeds.
Plant Physiol. 123, 497-508.
Fatland, B., Anderson, M., Nikolau, B.J. and Wurtele, E.S. (2000) Molecular
biology of cytosolic acetyl-CoA generation. Biochem. Soc. Trans.
28, 593-595.
Guan, X., Diez, T., Prasad, T.K., Nikolau, B.J. and Wurtele, E.S. (1999)
Geranoyl-CoA carboxylase: a novel biotin-containing enzyme in plants.
Arch. Biochem. Biophys. 362, 12-21.
Schnable, P.S., Hsia, A.P. and Nikolau, B.J. (1998) Genetic recombination
in plants. Curr. Opin. Plant Biol. 1, 123-129.
Liu, L., Hammond, E.G. and Nikolau, B.J. (1998) In vivo studies of the
biosynthesis of vernolic acid in the seed of Vernonia galamensis.
Lipids 33, 1217-1221.
Anderson, M.D., Che, P., Song, J., Nikolau, B.J. and Wurtele, E.S. (1998)
3-Methylcrotonyl-coenzyme A carboxylase is a compon
