- Plant Natural Products (Terpenoid) Biosynthesis Enzyme Mechanisms and Engineering
- Biochemical Pathway Identification (Functional Genomics)
- Metabolic Engineering
4216 Molecular Biology Building
Dept. of Biochemistry, Biophysics & Molecular Biology
Iowa State University
Ames, IA 50011
Phone: (515) 294-8580
B.S., Molecular Biology, UC San Diego, 1992
Ph.D., Biochemistry, UC San Francisco, 1998
Postdoctoral Fellow, Washington State University, 1998-2002
Research in the Peters laboratory is focused on the large class of diterpenoid natural products, largely those falling within the labdane-related superfamily. Bioactivity of these ~7,000 known compounds ranges from the gibberellic acid (GA) hormones ubiquitously found in higher/flowering plants to natural antibiotics, and includes compounds of pharmaceutical and industrial relevance. Their biosynthesis is unified in being initiated by a pair of terpene synthase catalyzed reactions. Beginning with a poorly understood acid/base catalyzed (class II) cyclization reaction, which forms the bicyclic core that defines this super-
family of compounds, and proceeding through a more typical allylic diphosphate ionization driven (class I) reaction. The
resulting cyclic hydrocarbon is generally then oxygenated via the action of cytochromes P450, with the ensuing compounds
often further elaborated by redox reactions and/or addition of various chemical groups by transferases.
The importance of GA for plant growth and development resulted in early investigations of their biosynthesis including identification of all the enzymes involved in GA metabolism from plants, as well as from the phytopathogenic fungus
Gibberella fujikuroi, from which GA was first isolated. We are engaged in mechanistic studies of the relevant enzymes from
the model plant species Arabidopsis thaliana. This provides deeper insights into this critical metabolic pathway, e.g. for
identification of potential biochemical regulatory mechanisms, and also provides a baseline against which the specificity of enzymes involved in alternative biosynthetic pathways can be measured. In addition, we have begun investigating the
production of GA by certain (rhizo)bacteria as well.
The absolute requirement for GA phytohormones in all flowering plants provides a reservoir of biosynthetic genes,
duplication of which (e.g., as a result of the whole genome duplications that are prevalent in plant evolutionary history
as well as gene duplication events) has enabled the derivation of alternative diterpenoid metabolism. For example, rice
(Oryza sativa) produces more than twenty labdane-related diterpenoid natural products, many of which act as natural
antibiotics against the devastating fungal blast pathogene Magneporthe oryzae. Underlying this rice phytochemical diversity
is a correspondingly complex metabolic network, and we are taking a functional genomics based approach to elucidate the enzymatic activity of potential diterpenoid biosynthetic genes from this model cereal crop plant. Moreover, our identification of the relevant enzymatic genes enables a reverse genetic approach to investigation of the biological relevance/activity
of the rice diterpenoids.
Finally, among the many labdane-related diterpenoid natural products are a number of compounds with significant potential to impact human health, ranging from potential and realized pharmaceuticals to a virulence factor in the human pathogen Mycobacterium tuberculosis. Accordingly, we are studying the biosynthesis of these natural products as well, which is
expected to both provide increased access to this large superfamily, through enzymatic and metabolic engineering efforts,
along with elucidating mechanistic features of potential drug targets against M. tuberculosis.
1: Zi J, Mafu S, Peters RJ. To Gibberellins and Beyond! Surveying the Evolution of (Di)Terpenoid Metabolism. Annu Rev Plant Biol. 2014 Jan 22. [Epub ahead of print] PubMed PMID: 24471837.
2: Hershey DM, Lu X, Zi J, Peters RJ. Functional conservation of the capacity for ent-kaurene biosynthesis and an associated operon in certain rhizobia. J Bacteriol. 2014 Jan;196(1):100-6. doi: 10.1128/JB.01031-13. Epub 2013 Oct 18. PubMed PMID: 24142247; PubMed Central PMCID: PMC3911121.
3: Zi J, Peters RJ. Characterization of CYP76AH4 clarifies phenolic diterpenoid biosynthesis in the Lamiaceae. Org Biomol Chem. 2013 Nov 28;11(44):7650-2. doi: 10.1039/c3ob41885e. Epub 2013 Oct 10. PubMed PMID: 24108414; PubMed Central PMCID: PMC3922537.
4: Wu Y, Wang Q, Hillwig ML, Peters RJ. Picking sides: distinct roles for CYP76M6 and CYP76M8 in rice oryzalexin biosynthesis. Biochem J. 2013 Sep 1;454(2):209-16. PubMed PMID: 23795884; PubMed Central PMCID: PMC3787970.
5: Criswell J, Potter K, Shephard F, Beale MH, Peters RJ. A single residue change leads to a hydroxylated product from the class II diterpene cyclization catalyzed by abietadiene synthase. Org Lett. 2012 Dec 7;14(23):5828-31. doi: 10.1021/ol3026022. Epub 2012 Nov 20. PubMed PMID: 23167845; PubMed Central PMCID: PMC3518578.
6: Mann FM, Xu M, Davenport EK, Peters RJ. Functional characterization and evolution of the isotuberculosinol operon in Mycobacterium tuberculosis and related Mycobacteria. Front Microbiol. 2012 Oct 12;3:368. doi: 10.3389/fmicb.2012.00368. eCollection 2012. PubMed PMID: 23091471; PubMed Central PMCID: PMC3470408. 7: Zhou K, Xu M, Tiernan M, Xie Q, Toyomasu T, Sugawara C, Oku M, Usui M, Mitsuhashi W, Chono M, Chandler PM, Peters RJ. Functional characterization of wheat ent-kaurene(-like) synthases indicates continuing evolution of labdane-related diterpenoid metabolism in the cereals. Phytochemistry. 2012 Dec;84:47-55. doi: 10.1016/j.phytochem.2012.08.021. Epub 2012 Sep 22. PubMed PMID: 23009879; PubMed Central PMCID: PMC3483413.
8: Wu Y, Zhou K, Toyomasu T, Sugawara C, Oku M, Abe S, Usui M, Mitsuhashi W, Chono M, Chandler PM, Peters RJ. Functional characterization of wheat copalyl diphosphate synthases sheds light on the early evolution of labdane-related diterpenoid metabolism in the cereals. Phytochemistry. 2012 Dec;84:40-6. doi: 10.1016/j.phytochem.2012.08.022. Epub 2012 Sep 23. PubMed PMID: 23009878; PubMed Central PMCID: PMC3483432.
9: Gao Y, Honzatko RB, Peters RJ. Terpenoid synthase structures: a so far incomplete view of complex catalysis. Nat Prod Rep. 2012 Oct;29(10):1153-75. doi: 10.1039/c2np20059g. Epub 2012 Aug 21. Review. PubMed PMID: 22907771; PubMed Central PMCID: PMC3448952.
10: Wang Q, Hillwig ML, Wu Y, Peters RJ. CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism. Plant Physiol. 2012 Mar;158(3):1418-25. doi: 10.1104/pp.111.187518. Epub 2012 Jan 12. PubMed PMID: 22247270; PubMed Central PMCID: PMC3291257.
11: Zhou K, Gao Y, Hoy JA, Mann FM, Honzatko RB, Peters RJ. Insights into diterpene cyclization from structure of bifunctional abietadiene synthase from Abies grandis. J Biol Chem. 2012 Feb 24;287(9):6840-50. doi: 10.1074/jbc.M111.337592. Epub 2012 Jan 4. PubMed PMID: 22219188; PubMed Central PMCID: PMC3307272.
12: Wang Q, Hillwig ML, Okada K, Yamazaki K, Wu Y, Swaminathan S, Yamane H, Peters RJ. Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster. J Biol Chem. 2012 Feb 24;287(9):6159-68. doi: 10.1074/jbc.M111.305599. Epub 2012 Jan 3. PubMed PMID: 22215681; PubMed Central PMCID: PMC3307284.
13: Xu M, Galhano R, Wiemann P, Bueno E, Tiernan M, Wu W, Chung IM, Gershenzon J, Tudzynski B, Sesma A, Peters RJ. Genetic evidence for natural product-mediated plant-plant allelopathy in rice (Oryza sativa). New Phytol. 2012 Feb;193(3):570-5. doi: 10.1111/j.1469-8137.2011.04005.x. Epub 2011 Dec 12. PubMed PMID: 22150231; PubMed Central PMCID: PMC3257406.
14: Hillwig ML, Xu M, Toyomasu T, Tiernan MS, Wei G, Cui G, Huang L, Peters RJ. Domain loss has independently occurred multiple times in plant terpene synthase evolution. Plant J. 2011 Dec;68(6):1051-60. doi: 10.1111/j.1365-313X.2011.04756.x. Epub 2011 Oct 17. PubMed PMID: 21999670; PubMed Central PMCID: PMC3237789. 15: Wu Y, Hillwig ML, Wang Q, Peters RJ. Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7. FEBS Lett. 2011 Nov 4;585(21):3446-51. doi: 10.1016/j.febslet.2011.09.038. Epub 2011 Oct 5. PubMed PMID: 21985968; PubMed Central PMCID: PMC3227696.
16: Mafu S, Hillwig ML, Peters RJ. A novel labda-7,13e-dien-15-ol-producing bifunctional diterpene synthase from Selaginella moellendorffii. Chembiochem. 2011 Sep 5;12(13):1984-7. doi: 10.1002/cbic.201100336. Epub 2011 Jul 12. PubMed PMID: 21751328; PubMed Central PMCID: PMC3383829.
17: Morrone D, Hillwig ML, Mead ME, Lowry L, Fulton DB, Peters RJ. Evident and latent plasticity across the rice diterpene synthase family with potential implications for the evolution of diterpenoid metabolism in the cereals. Biochem J. 2011 May 1;435(3):589-95. doi: 10.1042/BJ20101429. PubMed PMID: 21323642; PubMed Central PMCID: PMC3723722.
18: Zhou K, Peters RJ. Electrostatic effects on (di)terpene synthase product outcome. Chem Commun (Camb). 2011 Apr 14;47(14):4074-80. doi: 10.1039/c0cc02960b. Epub 2011 Feb 8. PubMed PMID: 21305070; PubMed Central PMCID: PMC3718459.
19: Mann FM, VanderVen BC, Peters RJ. Magnesium depletion triggers production of an immune modulating diterpenoid in Mycobacterium tuberculosis. Mol Microbiol. 2011 Mar;79(6):1594-601. doi: 10.1111/j.1365-2958.2011.07545.x. Epub 2011 Jan 25. PubMed PMID: 21244530; PubMed Central PMCID: PMC3084657.
20: Mann FM, Thomas JA, Peters RJ. Rv0989c encodes a novel (E)-geranyl diphosphate synthase facilitating decaprenyl diphosphate biosynthesis in Mycobacterium tuberculosis. FEBS Lett. 2011 Feb 4;585(3):549-54. doi: 10.1016/j.febslet.2011.01.007. Epub 2011 Jan 13. PubMed PMID: 21237161; PubMed Central PMCID: PMC3048469.