Edward Yu Publications

Assistant Professor
Joint appointment with Physics & Astronomy

Structural and mechanistic aspects of membrane transport
X-ray crystallography of membrane proteins
Biophysics

Selected Publications
Yu, E. W., J. R. Aires, G. McDermott, and H. Nikaido. 2005. A Periplasmic Drug-Binding Site of the AcrB Multidrug Efflux Pump: A Crystallographic and Site-Directed Mutagenesis Study. J. Bacteriol. 187:6804-6815
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Lu, W. C., C. Z. Wang, E. W. Yu, and K. M. Ho. 2006. Dynamics of the Trimeric AcrB Transporter Protein Inferred from a B-factor Analysis of the Crystal Structure. Proteins: Structure, Function, and Bioinformatics 62:152-158.

Su, C.-C., M. Li, R. Gu, Y. Takatsuka, G. McDermott, H. Nikaido, and E. W. Yu. 2006. Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay Pathway. J. Bacteriol. 188:7290-7296.

Li, M., X. Qiu, C.-C. Su, F. Long, R. Gu, G. McDermott, and E. W. Yu. 2006. Cloning, Expression, Purification, Crystallization and Preliminary X-ray Diffraction Analysis of the Regulator AcrR from Escherichia Coli. Acta Crystallogr. F62:1150-1152.

Su, C.-C., F. Shi, R. Gu, M. Li, G. McDermott, E. W. Yu, and Q. Zhang. 2007. Preliminary structural studies of the transcriptional regulator CmeR from Campylobacter jejuni. Acta Crystallogr. F63:34-36.

Su, C.-C., D. J. Rutherford, and E. W. Yu. 2007. Characterization of the multidrug efflux regulator AcrR from Escherichia coli. Biochem. Biophys. Res. Comm. 361:85-90.

Gu, R., C.-C. Su, F. Shi, M. Li, G. McDermott, Q. Zhang, and E. W. Yu. 2007. Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni. J. Mol. Biol. 372:583-593.

Li, M., R. Gu, C.-C. Su, M. D. Routh, K. C. Harris, E. S. Jewell, G. McDermott, and E. W. Yu. 2007. Crystal structure of the transcriptional regulator AcrR from Escherichia coli. J. Mol. Biol. 374:591-603.

Su, C.-C., and E. W. Yu. 2007. Ligand-transporter interaction in the AcrB multidrug efflux pump determined by fluorescence polarization assay. FEBS Lett. 581:4872-4976.

Su, C.-C., F. Long, G. McDermott, W. M. Shafer, and E. W. Yu. 2008. Crystallization and preliminary X-ray diffraction analysis of the multidrug efflux transporter NorM from Neisseria gonorrhoeae. Acta Crystallogr. F64:289-292.

Gu, R., M. Li, C.-C. Su, F. Long, M. D. Routh, F. Yang, G. McDermott, and E. W. Yu. 2008. Conformational change of the AcrR regulator reveals a possible mechanism of induction. Acta Cryst. F64:584-588.

Long, F., C. Rouquette-Loughlin, W. M. Shafer, and E. W. Yu. 2008. Functional cloning and characterization of the multidrug efflux pumps NorM from Neisseria gonorrhoeae and YdhE from Escherichia coli. Antimicrob. Agents Chemother. 52:3052-3060.

Yu, E.W., McDermott, G., Koshland, D.E., Jr. and Nikaido, H. (2004) Mechanism of drug transport in the AcrB multidrug efflux pump. Submitted for publication.

Yu, E.W., McDermott, G., Zgurskaya, H.I., Nikaido, H. and Koshland, D.E., Jr. (2003) Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump. Science 300, 976.

Yu, E.W., Aires, J.R. and Nikaido, H. (2003) AcrB multidrug efflux pump of Escherichia coli: A composite substrate-binding cavity of an exceptional flexibility generates its extremely wide substrate specificity. J. Bacteriol. 185, 5657.

Yu, E.W. and Koshland, D.E., Jr. (2001) Propagating conformational changes over long (and short) distances in proteins. Proc. Nat. Acad. Sci. USA 98, 9517.