Dr. Libuse Brachova

People
Scientist Alumni
Libuse Brachova

I am the Nikolau laboratory manager, with research interest in the biotin biosynthetic pathway.

Biotin is an essential cofactor for enzymes transferring carboxyl groups between substrates. The biotin biosynthetic pathway has not been fully elucidated in plants. The early steps leading to pimeloyl CoA remain unknown. The next step utilizing pimeloyl-CoA and alanine is catalyzed by enzyme 8-amino-7-oxononanoate synthese (KAPA), followed by 7,9-diaminopelargonic acid synthese, dethiobiotin synthetase, and biotin synthase. Our recent research is focused on characterizing a gene that may be involved in this biotin biosynthesis, a homolog of a gene that in E. coli is located in biotin operon, but its biochemical function remains unknown.

Publications:

Quanbeck SM, Brachova L, Campbell AA, Guan X, Perera A, He K, Rhee SY, Bais P, Dickerson JA, Dixon P, Wohlgemuth G, Fiehn O, Barkan L, Lange I, Lange BM, Lee I, Cortes D, Salazar C, Shuman J, Shulaev V, HuhmanDV, Sumner LW, Roth MR, Welti R, Ilarslan H, Wurtele ES and Nikolau BJ. 2012. Metabolomics as a hypothesis-generating functional genomics tool for the annotation of Arabidopsis thaliana genes of "unknown function." Frontiers in Plant Science 3:15. doi: 10.3389/fpls.2012.00015. eCollection 2012. PMID: 22645570.

Chen Y, Apolinario E, Brachova L, Kelman Z, Li Z, Nikolau BJ, Showman L, Sowers K, Orban J. 2011. A nuclear magnetic resonance based approach to accurate functional annotation of putative enzymes in the methanogen Methanosarcina acetivorans. BMC Genomics 12 Suppl 1:S7. doi: 10.1186/1471-2164-12-S1-S7. PMID: 21810209.

Li X, Ilarslan H, Brachova L, Qian HR, Li L, Che P, Wurtele ES, Nikolau BJ.  2011. Reverse genetic analysis of the two biotin-containing subunit genes of the heteromeric acetyl-CoA carboxylase indicates a unidirectional functional redundancy. Plant Physiology 155:293-314. doi: 10.1104/pp.110.165910. Epub 2010 Oct 28. PMID: 21030508.

Nikolau BJ, Perera MA, Brachova L, Shanks B. 2008. Platform biochemicals for a biorenewable chemical industry. Plant Journal 54(4):536-45. doi: 10.1111/j.1365-313X.2008.03484.x. PMID: 18476861.

Cha S, Zhang H, Ilarslan HI, Wurtele ES, Brachova L, Nikolau, BJ, Yeung, ES. 2008. Direct profiling and imaging of plant metabolites in intact tissues by using colloidal graphite-assisted laser desorption ionization mass spectrometry. Plant Journal 55(2):348-60. doi: 10.1111/j.1365-313X.2008.03507.x. Epub 2008 Apr 4. PMID: 18397372.

Hayden JM, Brachova L, Higgins K, Obermiller L, Sevanian A, Khandrika S, Reaven PD. 2002. Induction of monocyte differentiation and foam cell formation in vitro by 7-ketocholesterol. J Lipid Res. 43(1):26-35. PMID: 11792719

Lue LF, Walker DG, Brachova L, Beach TG, Rogers J, Schmidt AM, Stern DM, Yan SD. 2001. Involvement of microglial receptor for advanced glycation endproducts (RAGE) in Alzheimer's disease: identification of a cellular activation mechanism. Exp Neurol. 171(1):29-45. PMID: 11520119

Lue LF, Rydel R, Brigham EF, Yang LB, Hampel H, Murphy GM Jr, Brachova L, Yan SD, Walker DG, Shen Y, Rogers J. 2001. Inflammatory repertoire of Alzheimer's disease and nondemented elderly microglia in vitro. Glia. 35(1):72-9. PMID: 11424194

Lue LF, Kuo YM, Roher AE, Brachova L, Shen Y, Sue L, Beach T, Kurth JH, Rydel RE, Rogers J. 1999. Soluble amyloid beta peptide concentration as a predictor of synaptic change in Alzheimer's disease. Am J Pathol. 155(3):853-62. PMID: 10487842

Webster S, Lue LF, Brachova L, Tenner AJ, McGeer PL, Terai K, Walker DG, Bradt B, Cooper NR, Rogers J. 1997. Molecular and cellular characterization of the membrane attack complex, C5b-9, in Alzheimer's disease. Neurobiol Aging 18(4):415-21. PMID: 9330973

Lue LF, Brachova L, Civin WH, Rogers J. 1996. Inflammation, A beta deposition, and neurofibrillary tangle formation as correlates of Alzheimer's disease neurodegeneration. J Neuropathol Exp Neurol. 55(10):1083-8. PMID: 8858005

Rogers J, Webster S, Lue LF, Brachova L, Civin WH, Emmerling M, Shivers B, Walker D, McGeer P. 1996. Inflammation and Alzheimer's disease pathogenesis. Neurobiol Aging 17(5):681-6. Review. PMID: 8892340

Lue LF, Brachova L, Walker DG, Rogers J. 1996. Characterization of glial cultures from rapid autopsies of Alzheimer's and control patients. Neurobiol Aging 17(3):421-9. PMID: 8725904

Brachova L, Lue LF, Schultz J, el Rashidy T, Rogers J. 1993. Association cortex, cerebellum, and serum concentrations of C1q and factor B in Alzheimer's disease. Brain Res Mol Brain Res. 18(4):329-34. PMID: 8326828

Rogers J, Cooper NR, Webster S, Schultz J, McGeer PL, Styren SD, Civin WH, Brachova L, Bradt B, Ward P, et al. 1992. Complement activation by beta-amyloid in Alzheimer disease. Proc Natl Acad Sci USA 89(21):10016-20. PMID: 1438191

Rogers J, Schultz J, Brachova L, Lue LF, Webster S, Bradt B, Cooper NR, Moss DE. 1992. Complement activation and beta-amyloid-mediated neurotoxicity in Alzheimer's disease. Res Immunol. 143(6):624-30. Review. No abstract available. PMID: 1455054

Area of Expertise: 
Biotin Metabolism
Lab Management
Education: 
BS in Biochemistry, Charles University, Prague
MA in Biochemistry, Charles University, Prague
PhD in Biochemistry, Institute of Physiology, Czechoslovak Academy of Science, Prague.
Contact
515-294-0524
2252 Molecular Biology Building
Ames
IA
50011-1079