Characterization of the mitogen-regulated protein (proliferin) receptor


Publication Type:

Journal Article


Endocrinology, Volume 136, Number 1, p.283-8 (1995)


0013-7227 (Print)<br/>0013-7227 (Linking)

Accession Number:



Animals, Binding, Competitive, DNA/biosynthesis, Female, Gestational Age, Glycoproteins/pharmacology, Growth Substances/pharmacology, Intercellular Signaling Peptides and Proteins, Kinetics, Membranes/metabolism, Mice, Pregnancy, Receptors, Growth Factor/*metabolism, Uterus/metabolism


<p>Mitogen-regulated protein (MRP/PLF; also called proliferin) is a member of the PRL-GH family expressed by the placenta in mid-gestation. This report describes an MRP/PLF receptor in uterine membrane preparations from pregnant mice. Peak receptor activity occurred at 11 days of gestation with a dissociation constant of 6 x 10(-10) M and maximal binding capacity of 86 fmole MRP/PLF/mg membrane protein. PRL, PL-I, and mannose-6-phosphate did not compete for binding, and GH competed weakly only at high concentrations. Primary cells cultured from uteri taken at days 8-11 of gestation responded to MRP/PLF with increases in DNA synthesis. Uterine cells from later stages of gestation did not respond to MRP/PLF. This is the first reported evidence of a function mediated by MRP/PLF and suggests a role for this protein in maternal-fetal interactions during reproduction. Thus, it seems that MRP/PLF is a placentally derived growth factor, which stimulates proliferation in the uterus in a developmentally defined period to coordinate uterine growth with fetal development.</p>


Nelson, J T<br/>Rosenzweig, N<br/>Nilsen-Hamilton, M<br/>HD-29087/HD/NICHD NIH HHS/<br/>Endocrinology. 1995 Jan;136(1):283-8.